Identifying Novel Noncoding RNAs
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The human genome project has shown that only a small fraction (<2%) of human genome can be transcribed into mRNA that is further translated into protein, and the vast majority of the mammalian genome might express non-coding RNA (ncRNA). Although a number of long non-coding RNAs (lncRNAs) have been recently shown to play significant roles in the regulation of gene expression or protein activity in critical signaling pathways, the total number of ncRNAs and the fraction of functional ncRNAs within the mammalian genome are still mysteries. To reveal the landscape of ncRNA expression and specifically, to capture the expression of transient RNAs, we have developed an RNA-seq Analysis pipeline of Transcriptome Reconstruction and Annotation to Identify Novel non-coding RNAs from exosome deficient cells (ATRAIN).
“RNA-Exosome-Regulated Long Non-coding RNA Transcription Controls Super-Enhancer Activity”
AUTHORS: Evangelos Pefanis*, Jiguang Wang*, Gerson Rothschild*, Junghyun Lim*, et al.
LINK TO PUBLICATION:
Cell 2015 May; doi: 10.1016/j.cell.2015.04.034
*These authors have contributed equally to this work.